Gossypol Inhibition of Acrosin and Proacrosin, and Oocyte Penetration by Human Spermatozoa
نویسندگان
چکیده
منابع مشابه
Relationship between acrosin activity of human spermatozoa and oxidative stress.
AIM To study the association between seminal oxidative stress and human sperm acrosin activity. METHODS It is a prospective study consisting of 30 infertile men and 12 fertile normozoospermic volunteers. A full history, clinical examination and scrotal ultrasound were done to exclude other related factors such as smoking and varicocele. Presence of white blood cells (WBCs) in semen samples wa...
متن کاملOocyte penetration by fresh or stored diluted boar spermatozoa before and after in vitro capacitation treatments.
This study examined whether washing and preincubation of boar spermatozoa was necessary to achieve high in vitro penetration rates of pig oocytes. In experiment 1, diluted, sperm-rich fractions, stored for 24 h at 16 degrees C (stored diluted sperm) were used. After centrifugation once at 50 g for 3 min, the supernatant was concentrated at 1200 g for 3 min. The pellets were washed (1200 g for 3...
متن کاملProacrosin activation mechanisms in capacitated and frozen-thawed boar spermatozoa.
The main objective of the current study was to explore the different activation mechanisms of capacitation and freeze-thawed spermatozoa. Using SDS-PAGE and Western blotting, the conversion process of boar proacrosin during freeze-thawing and capacitation of spermatozoa was analyzed. The results revealed that capacitated spermatozoa exhibited a greater fluorescence area than that of the freeze-...
متن کاملProacrosin conversion inhibitor. Purification and initial characterization of a boar sperm protein which prevents the conversion of proacrosin into acrosin.
A proacrosin conversion inhibitor present in boar spermatozoa has been purified and initially characterized. Purification methods included sequential acid extractions of washed spermatozoa at pH 4.0, pH 3.5, and pH 2.5 followed by successive gel filtrations of the pH 2.5 sperm extract supernatant over Sephadex G-75 and G-50. The resulting 8.8-fold purified materials were judged to be homogeneou...
متن کاملDemonstration of acrosin in mouse spermatozoa.
Gelatinolytic activity of whole mouse spermatozoa was demonstrated by the gelatin film test. The presence of a trypsin-like protease (acrosin) in acidic extracts of mouse spermatozoa was shown by an electrophoretic method to separate the enzyme from a putative inhibitor.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1983
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod29.4.999